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Projects

0

Publications

42

Awards

0


Contact Details

Associate Professor

    Biography

    Research Overview

    The study of macromolecular interactions

    The group combines expertise in the functional and structural characterization of protein complexes and is particularly interested in the study of protein–ligand interactions. Proteins of interest to the group include: a) bacterial DNA replication proteins, transcription factors and metabolic enzymes; and b) viral nucleoproteins, replication proteins and integrases.

    Development of ultrasensitive immunodiagnostics

    This program involves the development of innovative diagnostic platforms. The group’s successes include the development of the TT-lock Immuno-PCR platform and its application for the diagnosis of melioidosis as well as a sensitive assay to detect the presence of the influenza A nucleoprotein and seafood allergy IgE. Currently the group focuses on developing a new rapid test platform technology for Ebola, influenza A/B, SARS-CoV-2, MERS and RSV.

    Drug discovery

    This program is in full swing and builds on recent successes in the development of innovative high-throughput assays using the principle of high-throughput differential scanning fluorimetry of GFP-tagged proteins. The group is interested in the identification of drug leads and their mechanisms of action. Diseases of particular interest to the group include: dengue, melioidosis, tuberculosis, malaria and HIV.

    Development of bio- and nanotechnologies

    The group has a long-standing interest and track-record in developing innovative sensing technologies based on fluorescence, biomolecular recognition and protein thermal stability.

    Expertise

    Protein Biochemistry; DNA replication; Protein-ligand interactions; Immuno-PCR diagnostics; Surface Plasmon Resonance; Protein unfolding and aggregation; Drug targets; High-throughput biotechnologies; GFP

    Teaching

    Research Advisor Accreditation
    Advisor Type

    Mentor

    Research Advisor Accreditation
    Role

    Chair; Independent Academic


    Current JCU Research Students
    Synthesis of GFP-tagged fibrillin-2 to study the pathogenesis of congenital contractural arachnodactyly (CCA)
    Master of Philosophy (Medical and Molecular Sciences)
    Characterising the Burkholderia pseudomallei replisome for future melioidosis drug discovery
    Master of Philosophy (Medical, Molecular and Veterinary Sciences)
    Defining the ancestral replication fork trap in Tus-dependent bacteria
    Doctor of Philosophy (Natural and Physical Sciences)

    Output

    Toft, Casey J.; Stocks, Bradley B.; Schaeffer, Patrick M. (2024) 'Comparison of the analytical sensi. Talanta, 275 . [DOI] ...
    Journal Publication ResearchOnline@JCU
    Toft, Casey J.; Bourquin, Rebecca A.; Sorenson, Alanna E.; Horwood, Paul F.; Druce, Julian; Schaeffe...
    Journal Publication ResearchOnline@JCU
    Sorenson, Alanna E.; Schaeffer, Patrick M. (2023) 'Real-Time Temperature Sensing Using a Ratiometric. Biosensors, 13 (3). [DOI] ...
    Journal Publication ResearchOnline@JCU
    Radford, Holly M.; Toft, Casey J.; Sorenson, Alanna E.; Schaeffer, Patrick M. (2023) 'Inhibition of . Internationa, 24 (10). ...
    Journal Publication ResearchOnline@JCU
    Toft, Casey J.; Sorenson, Alanna E.; Schaeffer, Patrick M. (2022) 'Rise of the terminator protein tu. Analytica Chimica Acta, 1213 . [DOI] ...
    Journal Publication ResearchOnline@JCU
    Toft, Casey J.; Sorenson, Alanna E.; Schaeffer, Patrick M. (2022) 'A soft Tus-Ter interaction is hid. Microbiological Research, 263 . [DOI] ...
    Journal Publication ResearchOnline@JCU
    Toft, Casey; Moreau, Morgane J.J.; Perutka, Jiri; Mandapati, Savitri; Enyeart, Peter; Sorenson, Alan...
    Journal Publication ResearchOnline@JCU
    Sorenson, A.E.; Schaeffer, P.M. (2020) 'A new bivalent fluorescent fusion protein for differential C. Analytica Chimica Acta, 1101 :120-128. [DOI] ...
    Journal Publication ResearchOnline@JCU
    Johnston, Elecia B.; Kamath, Sandip D.; Iyer, Swati P.; Pratap, Kunal; Karnaneedi, Shaymaviswanathan...
    Journal Publication ResearchOnline@JCU
    Razaghi, Ali; Heimann, Kirsten; Schaeffer, Patrick M.; Gibson, Spencer B. (2018) 'Negative regulator. Apoptosis, 23 (2):93-112....
    Journal Publication ResearchOnline@JCU
    Askin, Samuel; Bond, Thomas E.H.; Sorenson, Alanna E.; Moreau, Morgane J.J.; Antony, Helma; Davis, R...
    Journal Publication ResearchOnline@JCU
    Bond, Thomas E.H.; Sorenson, Alanna E.; Schaeffer, Patrick M. (2017) 'A green fluorescent protein-ba. Microbiological Research, 205 :35-39. [DOI] ...
    Journal Publication ResearchOnline@JCU
    Bond, Thomas E.H.; Sorenson, Alanna E.; Schaeffer, Patrick M. (2017) 'Functional characterisation of. Microbiological Research, 199 :40-48. [DOI] ...
    Journal Publication ResearchOnline@JCU
    Kamath, Sandip; Johnston, Elecia; Iyer, Swati; Schaeffer, Patrick; Koplin, Jennifer; Allen, Katrina;...
    Journal Publication ResearchOnline@JCU
    Askin, Samuel P.; Bond, Thomas E.H.; Schaeffer, Patrick M. (2016) 'Green fluorescent protein-based a. Analytical Methods, 8 (2):418-424. [DOI] ...
    Journal Publication ResearchOnline@JCU
    Sorenson, Alanna E.; Askin, Samuel P.; Schaeffer, Patrick M. (2015) 'In-gel detection of biotin–prot. Analytical Methods, 7 :2087-2092. [DOI] ...
    Journal Publication ResearchOnline@JCU
    Johnston, Elecia B.; Kamath, Sandip D.; Lopata, Andreas L.; Schaeffer, Patrick M. (2014) 'Tus-Ter-lo. Bioanaly, 6 (4):...
    Journal Publication ResearchOnline@JCU
    Sorenson, Alanna E.; Williams, Natasha L.; Morris, Jodie L.; Ketheesan, Natkunam; Norton, Robert E.;...
    Journal Publication ResearchOnline@JCU
    Moreau, Morgane J.J.; Schaeffer, Patrick M. (2013) 'Dissecting the salt dependence of the Tus-Ter pr. Molecular BioSystems, 9 (12):3146-3154. [DOI] ...
    Journal Publication ResearchOnline@JCU
    Antony, Helma; Schaeffer, Patrick M. (2013) 'A GFP-tagged nucleoprotein-based aggregation assay for . 138 (20):6073-6080. [DOI] ...
    Journal Publication ResearchOnline@JCU
    Cooper, Alanna; Williams, Natasha L.; Morris, Jodie L.; Norton, Robert E.; Ketheesan, Natkunam; Scha...
    Journal Publication ResearchOnline@JCU
    Moreau, Morgane J.J.; Schaeffer, Patrick M. (2012) 'Differential Tus–Ter binding and lock formation:. Molecular BioSystems, 8 (10):2783-2791. [DOI] ...
    Journal Publication ResearchOnline@JCU
    Moreau, Morgane J.J; Schaeffer, Patrick M. (2012) 'A polyplex qPCR-based binding assay for protein–D. Analyst, 137 :4111-4113. [DOI] ...
    Journal Publication ResearchOnline@JCU
    Morin, Isabelle; Schaeffer, Patrick M. (2012) 'Combining RNA-DNA swapping and quantitative polymeras. 420 (2):121-126. [DOI] ...
    Journal Publication ResearchOnline@JCU
    Askin, Samuel P.; Schaeffer, Patrick M. (2012) 'A universal immuno-PCR platform for comparative and . Analyst, 137 (22):5193-5196. [DOI] ...
    Journal Publication ResearchOnline@JCU
    Moreau, Morgane J.J.; Morin, Isabelle; Askin, Samuel P.; Cooper, Alanna; Moreland, Nicole J.; Vasude...
    Journal Publication ResearchOnline@JCU
    Morin, Isabelle; Askin, Samuel P.; Schaeffer, Patrick M. (2011) 'IgG-detection devices for the Tus-T. Analyst, 136 (22):4815-4821. [DOI] ...
    Journal Publication ResearchOnline@JCU
    Askin, Samuel P.; Morin, Isabelle; Schaeffer, Patrick M. (2011) 'Development of a protease activity . 415 (2):126-133. [DOI] ...
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    Morin, Isabelle; Dixon, Nicholas E.; Schaeffer, Patrick M. (2010) 'Ultrasensitive detection of antib. Molecular BioSystems, 6 (7):1173-1175. [DOI] ...
    Journal Publication ResearchOnline@JCU
    Moreau, Morgane J.J.; Morin, Isabelle; Schaeffer, Patrick M. (2010) 'Quantitative determination of p. Molecular BioSystems, 6 (7):1285-1292. [DOI] ...
    Journal Publication ResearchOnline@JCU
    Dahdah, Dahdah B.; Morin, Isabelle; Moreau, Morgane J.J.; Dixon, Nicholas E.; Schaeffer, Patrick M. ...
    Journal Publication ResearchOnline@JCU
    Schaeffer, Patrick; Dixon, Nicholas (2009) 'Synthesis and applications of covalent protein-DNA conju. Australian Journal of Chemistry, 62 (10):1328-1332. ...
    Journal Publication ResearchOnline@JCU
    Tanner, Nathan A.; Hamdan, Samir M.; Jergic, Slobodan; Loscha, Karin V.; Schaeffer, Patrick M.; Dixo...
    Journal Publication ResearchOnline@JCU
    Watt, Stephen J.; Urathamakul, Thitima; Schaeffer, Patrick M.; Williams, Neal K.; Sheil, Margaret M....
    Journal Publication ResearchOnline@JCU
    Su, Xun-Cheng; Schaeffer, Patrick M.; Loscha, Karin V.; Gan, Pamela H. P.; Dixon, Nicholas E. ; Otti...
    Journal Publication ResearchOnline@JCU
    Ioannou, Charikleia; Schaeffer, Patrick M.; Dixon, Nicholas E.; Soultanas, Panos (2006) 'Helicase bi. Nucleic A, 34 (18)...
    Journal Publication ResearchOnline@JCU
    Mulcair, Mark D.; Schaeffer, Patrick M.; Oakley, Aaron J.; Cross, Hannah F.; Neylon, Cameron; Hill, ...
    Journal Publication ResearchOnline@JCU
    Beck, Jennifer L.; Urathamakul, Thitima; Watt, Stephen J.; Sheil, Margaret M.; Schaeffer, Patrick M....
    Journal Publication ResearchOnline@JCU
    Graham, Stephen C.; Lilley, Penelope E.; Lee, Mihwa; Schaeffer, Patrick M.; Kralicek, Andrew V.; Dix...
    Journal Publication ResearchOnline@JCU
    Robinson, Andrew; Wu, Peter S.-C.; Harrop, Stephen J. ; Schaeffer, Patrick M. ; Dosztanyi, Zsuzsanna...
    Journal Publication ResearchOnline@JCU
    Schaeffer, Patrick M.; Headlam, Madeleine J.; Dixon, Nicholas E. (2005) 'Protein - protein interacti. 57 (1):5-12. [DOI] ...
    Journal Publication ResearchOnline@JCU
    Oakley, Aaron J.; Loscha, Karin V.; Schaeffer, Patrick M.; Liepinsh, Edvards; Pintacuda, Guido; Wilc...
    Journal Publication ResearchOnline@JCU