Differential Tus–Ter binding and lock formation: implications for DNA replication termination in Escherichia coli

Moreau, Morgane J.J.;Schaeffer, Patrick M.

Abstract

In E. coli, DNA replication termination occurs at Ter sites and is mediated by Tus. Two clusters of five Ter sites are located on each side of the terminus region and constrain replication forks in a polar manner. The polarity is due to the formation of the Tus–Ter-lock intermediate. Recently, it has been shown that DnaB helicase which unwinds DNA at the replication fork is preferentially stopped at the non-permissive face of a Tus–Ter complex without formation of the Tus–Ter-lock and that fork pausing efficiency is sequence dependent, raising two essential questions: Does the affinity of Tus for the different Ter sites correlate with fork pausing efficiency? Is formation of the Tus–Ter-lock the key factor in fork pausing? The combined use of surface plasmon resonance and GFP-Basta showed that Tus binds strongly to TerA–E and G, moderately to TerH–J and weakly to TerF. Out of these ten Ter sites only two, TerF and H, were not able to form significant Tus–Ter-locks. Finally, Tus's resistance to dissociation from Ter sites and the strength of the Tus–Ter-locks correlate with the differences in fork pausing efficiency observed for the different Ter sites by Duggin and Bell (2009).

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Molecular BioSystems

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1742-2051

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Royal Society of Chemistry

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DOI

10.1039/C2MB25281C