Ultrasensitive detection of antibodies using a new Tus-Ter-lock immunoPCR system

Journal Publication ResearchOnline@JCU
Morin, Isabelle;Dixon, Nicholas E.;Schaeffer, Patrick M.
Patrick Schaeffer
Abstract

A system consisting of a protein LG coated surface for the capture of mammalian antibodies (target), and an antigen fused to Tus and stoichiometrically linked to a DNA template via the Tus-Ter-lock sequence allowed the ultrasensitive detection of 5.5 attomol of target by real-time immunoPCR in complex media.

Journal

Molecular BioSystems

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Volume

6

ISBN/ISSN

1742-2051

Edition

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Issue

7

Pages Count

3

Location

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Publisher

Royal Society of Chemistry

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Publisher Location

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Publish Date

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Url

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Date

N/A

EISSN

N/A

DOI

10.1039/C002163F