Ultrasensitive detection of antibodies using a new Tus-Ter-lock immunoPCR system
Journal Publication ResearchOnline@JCUMorin, Isabelle;Dixon, Nicholas E.;Schaeffer, Patrick M.
Abstract
A system consisting of a protein LG coated surface for the capture of mammalian antibodies (target), and an antigen fused to Tus and stoichiometrically linked to a DNA template via the Tus-Ter-lock sequence allowed the ultrasensitive detection of 5.5 attomol of target by real-time immunoPCR in complex media.
Journal
Molecular BioSystems
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Volume
6
ISBN/ISSN
1742-2051
Edition
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Issue
7
Pages Count
3
Location
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Publisher
Royal Society of Chemistry
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Publisher Location
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Publish Date
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Date
N/A
EISSN
N/A
DOI
10.1039/C002163F