Projects 0
Publications 31
Awards 41

Contact Details

Adjunct Senior Lecturer
  • College of Science & Engineering, JCU Australia
Senior Research Fellow in Health Sciences and Food Safety
  • Tropical Futures Institute, JCU Singapore
Adjunct Scientist
  • The Bioinformatics Institute, Agency for Science, Technology and Research, Singapore

Biography

An early career researcher and science communicator with high expertise in medical sciences, protein-chemistry and clinical research, biochemical, immunological and analytical laboratory techniques, and thorough experience in leading interdisciplinary and international projects. He holds a PhD (cum laude) in Medical and Molecular Sciences from JCU (primary supervision by Prof. Andreas L. Lopata), and both Bachelor’s and Master’s degrees in Biochemistry from Heinrich Heine University of Düsseldorf (HHU, Germany) with specialised research training in Europe and the Asia-Pacific. His highest research expertise is in exploring food proteins and their role in allergic diseases. He is globally known for his work on fish allergy. In his pioneering PhD thesis and associated publications, he tackled this highly complex food allergy from the molecular to the global level. He evaluated the allergenicity (ability to trigger an allergic reaction) of more than 100 fish species and other related vertebrates, unravelling the multifaceted causes of fish allergies and identifying better ways to diagnose and manage this potentially deadly immune disease, which afflicts millions globally.

His primary research activities focus on molecular interactions between food and humans with an emphasis on the immune system. Aiming for translational collaborative research excellence, he is always interested in hearing from students keen on HDR projects, academics, companies and other stakeholders. Overall and foremost, Thimo seeks to help overcome challenges in securing humanity's (alternative) food protein needs under careful consideration of food safety, allergic diseases and planetary health.

Thimo is a Research Fellow at the Tropical Futures Institute (TFI, JCU, Singapore), Adjunct Scientist at The Bioinformatics Institute (BII, A*STAR, Singapore) and Adjunct Senior Lecturer at JCU Australia’s College of Science and Engineering (CSE), Australian Institute of Tropical Health and Medicine (AITHM), Centre for Molecular Therapeutics (CMT), Centre for Tropical Bioinformatics and Molecular Biology (CTBMB), and Centre for Sustainable Tropical Fisheries and Aquaculture (CSTFA). He is also a member of the Singapore Society for Mass Spectrometry (SMSS), Australia’s National Allergy Centre of Excellence Food Stream Advisory Group (NACE, only overseas member), Centre for Food Allergy Research (CFAR, first international scholar) - a Centre of Research Excellence by the Australian National Health and Medical Research Council (NHMRC), the World Allergy Organization (WAO), Asia Pacific Association of Allergy, Asthma and Clinical Immunology (APAAACI), Asia Pacific Association of Pediatric Allergy, Respirology and Immunology (APAPARI), European Academy of Allergy and Clinical Immunology (EAACI), American Academy of Allergy, Asthma and Immunology (AAAAI), Australian and New Zealand Society for Immunology (ASI), Australasian Society of Clinical Immunology and Allergy (ASCIA), Allergy & Anaphylaxis Australia (A&AA), and inVIVO Planetary Health Network (inVIVO).

He served as Guest editor for Foods and has been a reviewer for multiple journals including Allergy, Allergology International, Antibodies, Asian Pacific Journal of Allergy and Immunology, Clinical and Experimental Allergy, Food Chemistry, Food Control, Foods, Journal of Agricultural and Food Chemistry, Journal of the Science of Food and Agriculture, Molecular Immunology, and World Allergy Organization Journal.


Media and Stories - online, print, radio and TV

2026

2025

2024

2023

2022

2021

2020

2019

Research

Research Interests

Human and aquatic animal health

Improving tropical futures through translational research that has impact for people, environments and economies

Food allergies - Seafood allergies - Allergy Diagnostics and Management

Proteomics - Allergology – Immunology - Molecular medicine

Food safety - Sustainable food security

Promoting safe and healthy diets

Food authenticity and quality

Teaching

Teaching Interests

Training the next generation of scientists through translational collaborative research excellence


Research Advisor Accreditation

Advisor Type

Primary


Taught Modules

Current JCU Research Students

Profiling and identifying clinically cross-reactive allergens in shellfish and dust mite-sensitised patients.
Doctor of Philosophy (Medical, Molecular and Veterinary Sciences)
Seafood allergy and food innovations - Molecular and Immunological approaches
Doctor of Philosophy (Health)
Towards safer Seafood: Advancing Fish Allergy Diagnosis, Management and Risk Mitigation through Immunoproteomics
Doctor of Philosophy (Health)
Allergen profiles in Asian and American prawns for the development of improved diagnosis and immunotherapy
Doctor of Philosophy (Medical, Molecular and Veterinary Sciences)
The ecology, health and potential impacts of marine catfish (Family: Arridae) in Singapore near aquaculture sites.
Doctor of Philosophy (Agriculture, Environmental and Related Studies)

Completed JCU Research Students

Understanding Protein differences to make Healthier and Safer Seafood
Master of Philosophy (Health)- 2025

Activities

Awards

Associations

Employment

Output

Walsh, Ian; Ruethers, Thimo; Chiin, Sim Lyn; Teo, Gavin; Tay, Shi Jie; Wan, Corrine; Pang, Kuin Tian; Chia, Sean; Lopata, Andreas L.; Qiu, Beiying (2025) 'Differentiation of Fish Species Based on O-Acetylated N-Glycan Fragments Using LC-IM-MS to Combat Seafood Adulteration'. Applied Food Research, 5 (2). [DOI]
Journal Publication Open Access ResearchOnline@JCU
Dawes, Kathryn; Smart, Joanne; Ruethers, Thimo; Lopata, Andreas; Alhucema, Paulina; Campbell, Dianne E.; Ainsworth, John; Simpson, Bridget; Baldwin, Samara; Mahoney, Gabby; Baumgratner, JoJo; Mehr, Sam (2025) 'Gummy shark is tolerated by children with IgE-mediated fish allergy'. Journal Of Allergy And Clinical Immunology: In Practice, 13 (8):2186-2188. [DOI]
Journal Publication ResearchOnline@JCU
Heidari, Sahel; Ruethers, Thimo; Karnaneedi, Shaymaviswanathan; Yin, Lydia Wong Su; Lopata, Andreas (2025) 'Advances in Shellfish Allergy Therapy: From Current Approaches to Future Strategies'. Clinical Reviews in Allergy & Immunology, 68 . [DOI]
Journal Publication Open Access ResearchOnline@JCU
Limviphuvadh, Vachiranee; Ruethers, Thimo; Nguyen, Minh N.; Jerry, Dean R.; Smith, Benjamin P.C.; Wang, Yulan; Miao, Yansong; Andiappan, Anand Kumar; Lopata, Andreas L.; Maurer-Stroh, Sebastian (2025) 'Fish isoallergens and variants: database compilation, in silico allergenicity prediction challenges, and epitope-based threshold optimization'. Frontiers in Bioinformatics, 5 . [DOI]
Journal Publication Open Access ResearchOnline@JCU
Karnaneedi, Shaymaviswanathan; Johnston, Elecia B.; Bose, Utpal; Juhász, Angéla; Broadbent, James A.; Ruethers, Thimo; Jerry, Emily M.; Kamath, Sandip D.; Limviphuvadh, Vachiranee; Stockwell, Sally; Byrne, Keren; Clarke, Dean; Colgrave, Michelle L.; Maurer-Stroh, Sebastian; Lopata, Andreas L. (2024) 'The Allergen Profile of Two Edible Insect Species—Acheta domesticus and Hermetia illucens'. Molecular Nutrition & Food Research, 68 (16). [DOI]
Journal Publication Open Access ResearchOnline@JCU
Jerry, Emily M.; Karnaneedi, Shaymaviswanathan; Ruethers, Thimo; Jerry, Dean R.; Condon, Kelly; Lopata, Andreas L. (2024) 'Allergen Diversity and Abundance in Different Tissues of the Redclaw Crayfish (Cherax quadricarinatus)'. Foods, 13 (2). [DOI]
Journal Publication Open Access ResearchOnline@JCU
Dorney, Ryley D.; Johnston, Elecia B.; Karnaneedi, Shaymaviswanathan; Ruethers, Thimo; Kamath, Sandip D.; Gopi, Karthik; Mazumder, Debashish; Sammut, Jesmond; Jerry, Dean; Williamson, Nicholas A.; Nie, Shuai; Lopata, Andreas L. (2024) 'Variation in Shrimp Allergens: Place of Origin Effects on Food Safety Assessment'. International Journal of Molecular Sciences, 25 . [DOI]
Journal Publication Open Access ResearchOnline@JCU
Chin, Tak Gun Jeremy; Ruethers, Thimo; Chan, Bing Aleo; Lopata, Andreas Ludwig; Du, Juan (2024) 'Techno-functional properties and allergenicity of mung bean (Vigna radiata) protein isolates from Imara and KPS2 varieties'. Food Chemistry, 457 . [DOI]
Journal Publication Open Access ResearchOnline@JCU
Liu, Qing; Sui, Zengying; Feng, Nuan; Huang, Yuhao; Li, Yonghong; Ahmed, Ishfaq; Ruethers, Thimo; Liang, Hui; Li, Zhenxing; Lopata, Andreas L.; Sun, Lirui (2024) 'Characterization, Epitope Confirmation, and Cross-Reactivity Analysis of Parvalbumin from Lateolabrax maculatus by Multiomics Technologies'. Journal of Agricultural and Food Chemistry, 72 (36):20077-20090. [DOI]
Journal Publication Open Access ResearchOnline@JCU
Nugraha, Roni; Kurniawan, Fahmi; Abdullah, Asadatun; Lopata, Andreas L.; Ruethers, Thimo (2024) 'Antihypertensive and Antidiabetic Drug Candidates from Milkfish (Chanos chanos)—Identification and Characterization through an Integrated Bioinformatic Approach'. Foods, 13 (16). [DOI]
Journal Publication ResearchOnline@JCU
O'Malley, Andrea; Ray, Joshua M.; Kitlas, Patrycja; Ruethers, Thimo; Kapingidza, A. Brenda; Cierpicki, Tomasz; Lopata, Andreas; Kowal, Krzysztof; Chruszcz, Maksymilian (2024) 'Comparative studies of seafood and reptile α- and β-parvalbumins'. Protein Science, 33 (12). [DOI]
Journal Publication ResearchOnline@JCU
Taki, Aya C.; Ruethers, Thimo; Nugraha, Roni; Karnaneedi, Shaymaviswanathan; Williamson, Nicholas; Nie, Shuai; Leeming, Michael G.; Mehr, Sam S.; Campbell, Dianne E.; Lopata, Andreas L. (2023) 'Thermostable allergens in canned fish: Evaluating risks for fish allergy'. Allergy: European Journal of Allergy and Clinical Immunology, 78 (12):3221-3234. [DOI]
Journal Publication Open Access ResearchOnline@JCU
Ruethers, Thimo; Johnston, Elecia B.; Karnaneedi, Shaymaviswanathan; Nie, Shuai; Nugraha, Roni; Taki, Aya C.; Kamath, Sandip D.; Williamson, Nicholas A.; Mehr, Sam S.; Campbell, Dianne E.; Lopata, Andreas L. (2023) 'Commercial shellfish skin prick test extracts show critical variability in allergen repertoire'. Allergy, 78 (2):3261-3265. [DOI]
Journal Publication Open Access ResearchOnline@JCU
Ruethers, Thimo; Nugraha, Roni; Taki, Aya C.; O'Malley, Andrea; Karnaneedi, Shaymaviswanathan; Zhang, Stephanie; Kapingidza, A. Brenda; Mehr, Sam; Kamath, Sandip D.; Chruszcz, Maksymilian; Mackay, Graham; Campbell, Dianne E.; Lopata, Andreas L. (2022) 'The first reptilian allergen and major allergen for fish-allergic patients: Crocodile β-parvalbumin'. Pediatric Allergy and Immunology, 33 (5). [DOI]
Journal Publication Open Access ResearchOnline@JCU
Nugraha, Roni; Ruethers, Thimo; Taki, Aya C.; Johnston, Elecia B.; Karnaneedi, Shaymaviswanathan; Kamath, Sandip D.; Lopata, Andreas L. (2022) 'Recombinant Tropomyosin from the Pacific Oyster (Crassostrea gigas) for Better Diagnosis'. Foods, 11 (3). [DOI]
Journal Publication Open Access ResearchOnline@JCU
Sharp, Michael F.; Taki, Aya C.; Ruethers, Thimo; Stephen, Juan N.; Daly, Norelle L.; Lopata, Andreas L.; Kamath, Sandip D. (2021) 'IgE and IgG4 epitopes revealed on the major fish allergen Lat c 1'. Molecular Immunology, 131 :155-163. [DOI]
Journal Publication Open Access ResearchOnline@JCU
Nugraha, Roni; Ruethers, Thimo; Johnston, Elecia B.; Rolland, Jennifer M.; O’Hehir, Robyn E.; Kamath, Sandip D.; Lopata, Andreas L. (2021) 'Effects of extraction buffer on the solubility and immunoreactivity of the pacific oyster allergens'. Foods, 10 (2). [DOI]
Journal Publication Open Access ResearchOnline@JCU
Karnaneedi, Shaymaviswanathan; Huerlimann, Roger; Johnston, Elecia B.; Nugraha, Roni; Ruethers, Thimo; Taki, Aya C.; Kamath, Sandip D.; Wade, Nicholas M.; Jerry, Dean R.; Lopata, Andreas L. (2021) 'Novel allergen discovery through comprehensive de novo transcriptomic analyses of five shrimp species'. International Journal of Molecular Sciences, 22 (1). [DOI]
Journal Publication Open Access ResearchOnline@JCU
Heidelberg, Cecilie T.; Bang, Berit; Thomassen, Marte R.; Kamath, Sandip D.; Ruethers, Thimo; Lopata, Andreas L.; Madsen, Anne M.; Sandsund, Mariann; Aasmoe, Lisbeth (2021) 'Exposure to Bioaerosols during Fish Processing on Board Norwegian Fishing Trawlers'. Annals of Work Exposures and Health, 65 (6):694-702. [DOI]
Journal Publication Open Access ResearchOnline@JCU
Ruethers, Thimo; Taki, Aya C.; Karnaneedi, Shaymaviswanathan; Nie, Shuai; Kalic, Tanja; Dai, Danyi; Daduang, Sakda; Leeming, Michael; Williamson, Nicholas A.; Breiteneder, Heimo; Mehr, Sam S.; Kamath, Sandip; Campbell, Dianne E.; Lopata, Andreas (2021) 'Expanding the allergen repertoire of salmon and catfish'. Allergy, 76 (5):1443-1453. [DOI]
Journal Publication Open Access ResearchOnline@JCU
Ruethers, Thimo; Taki, Aya C.; Khangurha, Jasmit; Roberts, James; Buddhadasa, Saman; Clarke, Dean; Hedges, Claire E.; Campbell, Dianne E.; Kamath, Sandip D.; Lopata, Andreas L.; Koeberl, Martina (2020) 'Commercial fish ELISA kits have a limited capacity to detect different fish species and their products'. Journal of the Science of Food and Agriculture, 100 (12):4353-4363. [DOI]
Journal Publication Open Access ResearchOnline@JCU
Sheridan, Michael J.; Koeberl, Martina; Hedges, Claire Elizabeth; Biros, Erik; Ruethers, Thimo; Clarke, Dean; Buddhadasa, Saman; Kamath, Sandip; Lopata, Andreas Ludwig (2020) 'Undeclared allergens in imported packaged food for retail in Australia'. Food Additives & Contaminants: Part A, 37 (2):183-192. [DOI]
Journal Publication Open Access ResearchOnline@JCU
Kalic, Tanja; Kamath, Sandip D.; Ruethers, Thimo; Taki, Aya C.; Nugraha, Roni; Le, Thu T.K.; Humeniuk, Piotr; Williamson, Nicholas A.; Hira, Diamond; Rolland, Jennifer M.; O'Hehir, Robyn E.; Dai, Danyi; Campbell, Dianne E.; Breiteneder, Heimo; Lopata, Andreas L. (2020) 'Collagen-an important fish allergen for improved diagnosis'. Journal Of Allergy And Clinical Immunology: In Practice, 8 (9):3084-3092. [DOI]
Journal Publication Open Access ResearchOnline@JCU
Le, Thu T.K.; Nguyen, Duy H.; Vu, An T.L.; Ruethers, Thimo; Taki, Aya C.; Lopata, Andreas L. (2019) 'A cross-sectional, population-based study on the prevalence of food allergies among children in two different socio-economic regions of Vietnam'. Pediatric Allergy and Immunology, 30 (3):348-355. [DOI]
Journal Publication ResearchOnline@JCU
Kalic, Tanja; Effinger, Isabella; Kamath, Sandip D.; Palladino, Chiara; Mayr, Vanessa; Tscheppe, Angelika; Ruethers, Thimo; Waltl, Eva E.; Niederberger, Verena; Lengger, Nina; Radauer, Christian; Hafner, Christine; Lopata, Andreas L.; Bublin, Merima; Breiteneder, Heimo (2019) 'Fish-derived low molecular weight components modify bronchial epithelial barrier properties and release of pro-inflammatory cytokines'. Molecular Immunology, 112 :140-150. [DOI]
Journal Publication Open Access ResearchOnline@JCU
Nugraha, Roni; Kamath, Sandip D.; Johnston, Elecia; Karnaneedi, Shaymaviswanathan; Ruethers, Thimo; Lopata, Andreas L. (2019) 'Conservation analysis of B-cell allergen epitopes to predict clinical cross-reactivity between shellfish and inhalant invertebrate allergens'. Frontiers in Immunology, 10 . [DOI]
Journal Publication Open Access ResearchOnline@JCU
Kalic, Tanja; Morel-Codreanu, Francoise; Radauer, Christian; Ruethers, Thimo; Taki, Aya C.; Swoboda, Ines; Higer, Christiane; Hoffmann-Sommergruber, Karin; Ollert, Markus; Hafner, Christine; Lopata, Andreas L.; Morisset, Martine; Breiteneder, Heimo; Kuehn, Annette (2019) 'Patients allergic to fish tolerate ray based on the low allergenicity of its parvalbumin'. Journal Of Allergy And Clinical Immunology: In Practice, 7 (2):500-508. [DOI]
Journal Publication Open Access ResearchOnline@JCU
Ruethers, Thimo; Taki, Aya C.; Nugraha, Roni; Cao, Truc T.; Koeberl, Martina; Kannath, Sandip D.; Williamson, Nicholas A.; O'Callaghan, Sean; Nie, Shuai; Mehr, Sam S.; Cannpbell, Dianne E.; Lopata, Andreas L. (2019) 'Variability of allergens in commercial fish extracts for skin prick testing'. Allergy, 74 (7):1352-1363. [DOI]
Journal Publication ResearchOnline@JCU
Ruethers, T.; Raith, M.; Sharpe, M.F.; Koeberl, M.; Stephen, J.N.; Nugraha, R.; Le, T.T.K.; Quirce, S.; Nguyen, H.X.M.; Kamath, S.D.; Mehr, S.S.; Campbell, D.E.; Bridges, C.R.; Taki, A.C.; Swoboda, I.; Lopata, A.L. (2018) 'Characterization of Ras k 1 a novel major allergen in Indian mackerel and identification of parvalbumin as the major fish allergen in 33 Asia-Pacific fish species'. Clinical & Experimental Allergy, 48 (4):452-463. [DOI]
Journal Publication ResearchOnline@JCU
Ruethers, Thimo; Taki, Aya C.; Johnston, Elecia B.; Nugraha, Roni; Le, Thu T.K.; Kalic, Tanja; McLean, Thomas R.; Kamath, Sandip D.; Lopata, Andreas L. (2018) 'Seafood allergy: a comprehensive review of fish and shellfish allergens'. Molecular Immunology, 100 :28-57. [DOI]
Journal Publication ResearchOnline@JCU
Stephen, J.N.; Sharp, M.F.; Ruethers, T.; Taki, A.; Campbell, D.E.; Lopata, A.L. (2017) 'Allergenicity of bony and cartilaginous fish – molecular and immunological properties'. Clinical & Experimental Allergy, 47 (3):300-312. [DOI]
Journal Publication ResearchOnline@JCU