Commercial shellfish skin prick test extracts show critical variability in allergen repertoire

Ruethers, Thimo;Johnston, Elecia B.;Karnaneedi, Shaymaviswanathan;Nie, Shuai;Nugraha, Roni;Taki, Aya C.;Kamath, Sandip D.;Williamson, Nicholas A.;Mehr, Sam S.;Campbell, Dianne E.;Lopata, Andreas L.

Thimo Ruethers Shaymaviswanathan Karnaneedi Sandip Kamath Ludwig Lopata

Abstract

[Extract] Crustacean and mollusc (shellfish) allergy affects up to 3% of the general population, is usually lifelong and commonly triggers anaphylaxis.1 Allergen repertoire diversity among hundreds of edible shellfish species worldwide is poorly reflected in available in vivo and in vitro diagnostic tools for shellfish allergy. Skin prick testing (SPT) is often the preferred first-line diagnostic approach. However, widely utilized commercial SPT extracts are generally not standardized, limiting the diagnostic value of results.2 Asero et al. reported a heterogeneous abundance of three shellfish allergens in five commercial crustacean SPT extracts, resulting in 32 clinical profiles among 157 shrimp-allergic patients.3 In 2019, we demonstrated considerable variability in allergen repertoire and IgE-binding for 27 commercial fish SPT extracts.4 We now report an even greater, critical variability for 11 commercial crustacean and five mollusc SPT extracts, utilizing biochemical and immunological methods and mass spectrometry (see Appendix S1 for methodology and TableS1 for allergen extract details).

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1398-9995

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Wiley-Blackwell

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DOI

10.1111/all.15853