Screening and Selection of Potential Probiotic Bacteria from Mangrove Sediments

The increasing demand for freshwater fish has encouraged the development of intensive aquaculture systems with high stocking density, often leading to nutrient competition, disease outbreaks, and reduced growth performance. Probiotics from natural sources offer a potential solution to these challenges, including those isolated from mangrove sediments at Cemara Beach, Banyuwangi. This study aimed to identify and screen potential probiotic bacteria from these sediments by employing comprehensive functional and molecular approaches. Among the four isolates (SAX1, SBY1, SBY2, and SCZ1), SBY2 showed the most favorable probiotic profile, characterized by strong digestive enzyme production, broad environmental tolerance, and the largest inhibition zone against Aeromonas hydrophila among the tested isolates. After functional screening, molecular identification was conducted through DNA barcoding of the 16 S rRNA gene. Sequencing and phylogenetic analysis revealed that isolate SBY2 clustered within the Bacillus genus, showing high sequence similarity to Bacillus cereus, with the phylogenetic tree confirming its taxonomic position among reference strains. These results underscore the potential of B. cereus SBY2 as a candidate probiotic for aquaculture applications, offering multifunctional benefits for host health and culture system sustainability.

Thalassas

Thalassas

42

2366-1674

N/A

N/A

14