Successful cryopreservation of African wild dog (Lycaon pictus) spermatozoa: towards developing the frozen zoo

Conference Contribution ResearchOnline@JCU
Van den Berghe, F.;Paris, M.C.J.;Sarnyai, Z.;Briggs, M.B.;Farstad, W.K.;Paris, D.B.B.P.
Zoltan SarnyaiDamien Paris
Abstract

Sperm freezing and artificial insemination can aid species management and conservation of the African wild dog (Lycaon pictus). Freezing attempts have previously been unsuccessful with sperm motility dropping to nearly 0% within 2 h of thawing. We examined the quality of wild dog spermatozoa subjected to 2 routine canine cryopreservation protocols: 1) 1-step dilution in TRIS-egg yolk extender containing 8% glycerol and 20% egg yolk; and 2) 2-step dilution in TRIS-egg yolk extender to a final concentration of 5% glycerol, 20% egg yolk and 0.5% Equex STM. Protocol 2 showed a significantly higher post-thaw viability, acrosome integrity and longevity of spermatozoa with motility present for up to 8 h after thawing; making it suitable for sperm banking and downstream use in species management by artificial insemination for the first time.

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EAZA 2017: European Association of Zoos and Aquariums Annual Conference

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1

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Emmen, The Netherlands

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European Association of Zoos and Aquariums

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Emmen, The Netherlands

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