Characterization of polymorphic microsatellite loci for the spiny lobster Panulirus spp. and their utility to be applied to other Panulirus lobsters

Journal Publication ResearchOnline@JCU
Dao, Hoc Tan;Todd, Erica V.;Jerry, Dean R.
Dean Jerry
Abstract

The ornate spiny lobster Panulirus ornatus is a commercially important fishery and aquaculture species throughout south-east Asia and Australia. Thirty microsatellite markers were developed for P. ornatus of which 15 loci were consistently amplified and exhibited adequate levels of polymorphism for parentage and population genetic applications. Number of alleles observed per locus ranged from 2 to 23 (mean NA = 9.1 ± 1.4). Observed and expected heterozygosities ranged from 0.03–0.98 (HO mean = 0.61 ± 0.06) to 0.03–0.94 (HE mean = 0.66 ± 0.06), respectively. Microsatellite markers were also tested in three related Panulirus species, P. homarus, P. versicolor, and P. argus, with varying levels of amplification success and polymorphism exhibited.

Journal

Conservation Genetics Resources

Publication Name

Conservation Genetics Resources

Volume

5

ISBN/ISSN

1877-7260

Edition

N/A

Issue

1

Pages Count

4

Location

N/A

Publisher

Springer

Publisher Url

N/A

Publisher Location

N/A

Publish Date

N/A

Url

N/A

Date

N/A

EISSN

N/A

DOI

10.1007/s12686-012-9728-0