Free-living amoebae recovered from human stool samples in Strongyloides agar culture

Journal Contribution ResearchOnline@JCU
Bradbury, Richard, S.
Abstract

[Extract] Our laboratory in The Gambia, West Africa, performs Koga agar (1.5% bacteriological agar, 0.5% sodium chloride, 0.5% meat extract, 0.1% bacteriological peptone) culture for larvae of strongyle nematodes on human fecal samples for which parasitological investigation has been requested. We have recovered free-living amoebae (FLA) from human fecal specimens on two occasions over a period of 9 months (representing 130 individual fecal cultures) on this agar (Fig. 1). The amoebae in both cases were identified as Hartmannella species based upon the morphology of trophozoites and cysts in agar culture (Fig. 2) and their inability to enflagellate in distilled water after 8 h of incubation at 37°C.

Journal

Journal of Clinical Microbiology

Publication Name

N/A

Volume

52

ISBN/ISSN

1098-660X

Edition

N/A

Issue

2

Pages Count

2

Location

N/A

Publisher

American Society for Microbiology

Publisher Url

N/A

Publisher Location

N/A

Publish Date

N/A

Url

N/A

Date

N/A

EISSN

N/A

DOI

10.1128/JCM.02738-13