Tumor-promoting role of signal transducer and activator of transcription (Stat)1 in late-stage melanoma growth

Journal Publication ResearchOnline@JCU
Schultz, Julia;Koczan, Dirk;Schmitz, Ulf;Ibrahim, Saleh M.;Pilch, Dominik;Landsberg, Jenny;Kunz, Manfred
Abstract

A large-scale gene expression study of melanoma metastases was performed to identify genes involved in late-stage tumor progression. Overall 248 genes, out of more than 47,000 tested, are differentially expressed when comparing peripheral areas (invasion front) with central tumor areas of melanoma metastases. As determined by gene ontology analysis, members of the STAT signaling pathway show significant enrichment. In particular, Stat1 is highly expressed in peripheral compared with central tumor areas. In line with this, stable knockdown of STAT1 in metastatic melanoma cells significantly impairs their migratory and invasive capacity in wounding and matrigel assays. Moreover, STAT1 knockdown affects the metastatic behavior of melanoma cells in a mouse model of melanoma metastasis. Taken together, these data suggest that Stat1 might play a role in late-stage melanoma progression. Interference with the Stat1 pathway could have therapeutic implications for late-stage melanoma patients.

Journal

Clinical and Experimental Metastasis

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27

ISBN/ISSN

1573-7276

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Pages Count

8

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Publisher

Springer

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DOI

10.1007/s10585-010-9310-7