The immune checkpoint TIGIT is upregulated on T cells during bacterial infection and is a potential target for immunotherapy

McCulloch, Timothy R.;Rossi, Gustavo R.;Miranda-Hernandez, Socorro;Valencia-Hernandez, Ana Maria;Alim, Louisa;Belle, Clemence J.;Krause, Andrew;Zacchi, Lucia F.;Lam, Pui Yeng;Nakamura, Kyohei;Kupz, Andreas;Wells, Timothy J.;Souza-Fonseca-Guimaraes, Fernando

Ana Maria Valencia Hernandez

Abstract

Antibiotic resistance is a major public health threat, and alternatives to antibiotic therapy are urgently needed. Immunotherapy, particularly the blockade of inhibitory immune checkpoints, is a leading treatment option in cancer and autoimmunity. In this study, we used a murine model of Salmonella Typhimurium infection to investigate whether immune checkpoint blockade could be applied to bacterial infection. We found that the immune checkpoint T-cell immunoglobulin and ITIM domain (TIGIT) was significantly upregulated on lymphocytes during infection, particularly on CD4+ T cells, drastically limiting their proinflammatory function. Blockade of TIGIT in vivo using monoclonal antibodies was able to enhance immunity and improve bacterial clearance. The efficacy of anti-TIGIT was dependent on the capacity of the antibody to bind to Fc (fragment crystallizable) receptors, giving important insights into the mechanism of anti-TIGIT therapy. This research suggests that targeting immune checkpoints, such as TIGIT, has the potential to enhance immune responses toward bacteria and restore antibacterial treatment options in the face of antibiotic resistance.

Journal

Immunology and Cell Biology

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102

ISBN/ISSN

1440-1711

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Publisher

Nature Publishing Group

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DOI

10.1111/imcb.12794