Evaluation of Two Multiplex PCR–High-Resolution Melt Curve Analysis Methods for Differentiation of Campylobacter jejuni and Campylobacter coli Intraspecies
Journal Publication ResearchOnline@JCUAbstract
Campylobacter infection is a common cause of bacterial gastroenteritis in humans and remains a significant global public health issue. The capability of two multiplex PCR (mPCR)–high-resolution melt (HRM) curve analysis methods (i.e., mPCR1-HRM and mPCR2-HRM) to detect and differentiate 24 poultry isolates and three reference strains of Campylobacter jejuni and Campylobacter coli was investigated. Campylobacter jejuni and C. coli were successfully differentiated in both assays, but the differentiation power of mPCR2-HRM targeting the cadF gene was found superior to that of mPCR1-HRM targeting the gpsA gene or a hypothetical protein gene. However, higher intraspecies variation within C. coli and C. jejuni isolates was detected in mPCR1-HRM when compared with mPCR2-HRM. Both assays were rapid and required minimum interpretation skills for discrimination between and within Campylobacter species when using HRM curve analysis software.
Journal
Avian Diseases
Publication Name
N/A
Volume
62
ISBN/ISSN
1938-4351
Edition
N/A
Issue
1
Pages Count
8
Location
N/A
Publisher
American Association of Avian Pathologists
Publisher Url
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Publisher Location
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Publish Date
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Url
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Date
N/A
EISSN
N/A
DOI
10.1637/11739-080417-Reg.1