Best practice guidelines for environmental DNA biomonitoring in Australia and New Zealand

Journal Publication ResearchOnline@JCU
De Brauwer, Maarten;Clarke, Laurence J.;Chariton, Anthony;Cooper, Madalyn K.;De Bruyn, Mark;Furlan, Elise;Macdonald, Anna J.;Rourke, Meaghan L.;Sherman, Craig D.H.;Suter, Leonie;Villacorta Rath, Cecilia;Zaiko, Anastasija;Trujillo-González, Alejandro
Abstract

Environmental DNA (eDNA)- based methods are increasingly used by government agencies to detect pests and threatened species, and for broader biodiversity monitor-ing. Given rapid technological advances and a growing number of commercial service providers, there is a need to standardize methods for quality assurance and to main-tain confidence in eDNA- based results. Here, we introduce two documents to pro-vide best- practice guidelines for Australian and New Zealand eDNA researchers and end- users (available from https://sedna socie ty.com/publications ): the Environmental DNA protocol development guide for biomonitoring provides minimum standard consid-erations for eDNA and environmental RNA projects across the complete workflow, from ethical considerations and experimental design to interpreting and communicat-ing results. The Environmental DNA test validation guidelines outline key steps to be used in assay development and validation for species-specific testing and metabar-coding. Both guidelines were developed as an initiative of the Australian Government Department of Agriculture, Fisheries and Forestry and led by the Southern eDNA Society in a collaborative process including multiple consultation rounds with eDNA experts, end-users, and stakeholders to adapt the guidelines to Australian and New Zealand needs. The aim of these guidelines is not to be prescriptive, but to set mini-mum standards to support a consistent and best- practice approach to eDNA testing. We anticipate that the guidelines will be reviewed and regularly updated as required. Our aspiration is that these best- practice guidelines will ensure environmental man-agers are provided with robust scientific evidence to support decision- making.

Journal

Environmental DNA

Publication Name

N/A

Volume

5

ISBN/ISSN

2637-4943

Edition

N/A

Issue

3

Pages Count

7

Location

N/A

Publisher

John Wiley & Sons Ltd

Publisher Url

N/A

Publisher Location

N/A

Publish Date

N/A

Url

N/A

Date

N/A

EISSN

N/A

DOI

10.1002/edn3.395