Root Canal Microbiome Associated with Asymptomatic Apical Periodontitis

Conference Contribution ResearchOnline@JCU
Rodrigues Amaral, Rodrigo;Braga, Tiago;Siqueira, Jose F.;Rocas, Isabela N.;Coelho da Costa Rachid, Caio Tavora;Guimarães Oliveira, Anna Gabriella;Ilma de Souza Cortes, Maria;Love, Robert Mathew
Abstract

Root canal samples from 25 patients (ethics committee approval CAAE 9713 1918 7 0000 5137 )were prepared for DNA sequencing analysis Inclusion criteria were patients presenting no systemic disease, teeth with a single root and a single canal presenting with a carious lesion with intact pulp chamber walls, pulp necrosis confirmed by pulp sensibility tests, and clinical and radiographic evidence of asymptomatic primary apical periodontitis with no sinus tract. Bacterial diversity of the microbiome was identified and compared between cases and to the size of related periapical lesions Statistical analysis of the operational taxonomic unit distribution ( was performed using PCoA with Bray Curtis distance and PCoA Two Way Permanova Fisher Exact test was used to evaluate the prevalence of different OTUs related to small and large periapical lesions.

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ANZ IADR 61st Scientific Meeting

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1

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Melbourne, VIC, Australia

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International Association for Dental Research (IADR)

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Melbourne, VIC, Australia

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