A lineage-specific Exo70 is required for receptor kinase–mediated immunity in barley

Holden, Samuel;Bergum, Molly;Green, Phon;Bettgenhaeuser, Jan;Hernandez-Pinzon, Inmaculada;Thind, Anupriya;Clare, Shaun;Russell, James M.;Hubbard, Amelia;Taylor, Jodi;Smoker, Matthew;Gardiner, Matthew;Civolani, Laura;Cosenza, Francesco;Rosignoli, Serena;Strugala, Roxana;Molnar, István;Simkova, Hana;Dolezel, Jaroslav;Schaffrath, Ulrich;Barrett, Matthew;Salvi, Silvio;Moscou, Matthew J.

Matt Barrett

Abstract

In the evolution of land plants, the plant immune system has experienced expansion in immune receptor and signaling pathways. Lineage-specific expansions have been observed in diverse gene families that are potentially involved in immunity but lack causal association. Here, we show that Rps8-mediated resistance in barley to the pathogen Puccinia striiformis f. sp. tritici (wheat stripe rust) is conferred by a genetic module: Pur1 and Exo70FX12, which are together necessary and sufficient. Pur1 encodes a leucine-rich repeat receptor kinase and is the ortholog of rice Xa21, and Exo70FX12 belongs to the Poales-specific Exo70FX clade. The Exo70FX clade emerged after the divergence of the Bromeliaceae and Poaceae and comprises from 2 to 75 members in sequenced grasses. These results demonstrate the requirement of a lineage-specific Exo70FX12 in Pur1-mediated immunity and suggest that the Exo70FX clade may have evolved a specialized role in receptor kinase signaling.

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Science Advances

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2375-2548

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American Association for the Advancement of Science

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DOI

10.1126/sciadv.abn7258