A potent vaccine delivery system
Journal Contribution ResearchOnline@JCUAbstract
Most vaccines require co-delivery of an adjuvant in order to generate the desired immune responses. However, many currently available adjuvants are non-biodegradable, have limited efficacy, and/or poor safety profile. Thus, new adjuvants, or self-adjuvanting vaccine delivery systems, are required. Here, we proposed a self-adjuvanting delivery system that is fully defined, biodegradable, and non-toxic. The system is produced by conjugation of polyleucine to peptide antigen, followed by self-assembly of the conjugate into nanoparticles. The protocol includes solid-phase peptide synthesis of the vaccine conjugate, purification, self-assembly and physicochemical characterization of the product. Overall, this protocol describes, in detail, the production of a well-defined and effective self-adjuvanting delivery system for peptide antigens, along with tips for troubleshooting.
Journal
Bio-protocol
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Volume
11
ISBN/ISSN
2331-8325
Edition
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Issue
7
Pages Count
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Location
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Publisher
Bio-protocol
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Publisher Location
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Url
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Date
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EISSN
N/A
DOI
10.21769/BioProtoc.3973