Comparative analysis of plastid genomes in the non-photosynthetic genus Thismia reveals ongoing gene set reduction

Journal Publication ResearchOnline@JCU
Yudina, Sophia V.;Schelkunov, Mikhail I.;Nauheimer, Lars;Crayn, Darren;Chantanaorrapint, Sahut;Hroneš, Michal;Sochor, Michal;Dančák, Martin;Mar, Shek-Shing;Luu, Hong Truong;Nuraliev, Maxim S.;Logacheva, Maria D.
Abstract

Heterotrophic plants provide intriguing examples of reductive evolution. This is especially evident in the reduction of their plastid genomes, which can potentially proceed toward complete genome loss. Several milestones at the beginning of this path of degradation have been described; however, little is known about the latest stages of plastome reduction. Here we analyze a diversity of plastid genomes in a set of closely related non-photosynthetic plants. We demonstrate how a gradual loss of genes shapes the miniaturized plastomes of these plants. The subject of our study, the genus Thismia, represents the mycoheterotrophic monocot family Thismiaceae, a group that may have experienced a very ancient (60–80 mya) transition to heterotrophy. In all 18 species examined, the plastome is reduced to 14–18 kb and is highly AT-biased. The most complete observed gene set includes accD, seven ribosomal protein genes, three rRNA, and two tRNA genes. Different clades of Thismia have undergone further gene loss (complete absence or pseudogenization) compared to this set: in particular, we report two independent losses of rps2 and rps18.

Journal

Frontiers in Plant Science

Publication Name

N/A

Volume

12

ISBN/ISSN

1664-462X

Edition

N/A

Issue

N/A

Pages Count

13

Location

N/A

Publisher

Frontiers Research Foundation

Publisher Url

N/A

Publisher Location

N/A

Publish Date

N/A

Url

N/A

Date

N/A

EISSN

N/A

DOI

10.3389/fpls.2021.602598