Local and systemic inflammation and oxidative stress after a single bout of maximal walking in patients with symptomatic peripheral artery disease

Journal Publication ResearchOnline@JCU
Andrade-Lima, Aluisio;Da Silva Junior, Natan;Chehuen, Marcel;Miyasato, Roberto;Souza, Rodrigo W.A.;Leicht, Anthony S.;Brum, Patricia C.;De Oliveira, Edilamar M.;Wolosker, Nelson;Forjaz, Cláudia L.M.
Anthony Leicht
Abstract

Objective: The aim of this study was to assess the effects of a single bout of maximal walking on blood and muscle nitric oxide (NO) bioavailability, oxidative stress, and inflammation in symptomatic peripheral artery disease (PAD) patients. Methods: A total of 35 men with symptomatic PAD performed a graded maximal exercise test on a treadmill (3.2 km/h, 2% increase in grade every 2 minutes). Plasma samples and gastrocnemius muscle biopsies were collected preexercise and postexercise for assessment of NO bioavailability (plasma NO and muscle, endothelial NO synthase), oxidative stress and antioxidant function (lipid peroxidation [LPO], catalase [CAT], and superoxide dismutase), and inflammation (interleukin-6, C-reactive protein, tumor necrosis factor-α, intercellular adhesion molecules, and vascular adhesion molecules). The effects of the walking exercise were assessed using paired t tests or Wilcoxon tests. Results: After maximal walking, plasma NO and LPO were unchanged (P > .05), plasma CAT decreased, and all blood inflammatory markers increased (all P ≤ .05). In the disease-affected skeletal muscle, endothelial NO synthase, CAT, LPO, and all inflammatory markers increased, whereas superoxide dismutase decreased (all P ≤ .05). Conclusion: In patients with symptomatic PAD, maximal exercise induces local and systemic impairments, which may play a key role in atherogenesis. Exercise strategies that avoid maximal effort may be important to reduce local and systemic damage and enhance clinical benefits.

Journal

Journal of Cardiovascular Nursing

Publication Name

Journal of Cardiovascular Nursing

Volume

36

ISBN/ISSN

1550-5049

Edition

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Issue

5

Pages Count

9

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Publisher

Lippincott Williams & Wilkins

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EISSN

N/A

DOI

10.1097/JCN.0000000000000686