Identification of two distinct bovine parainfluenza virus type 3 genotypes

Horwood, Paul Francis;Gravel, Jennifer Lillian;Mahony, Timothy John

Abstract

The partial gene sequencing of the matrix (M) protein from seven clinical isolates of bovine parainfluenza virus type 3 (BPIV-3), and the complete sequencing of a representative isolate (Q5592) was completed in this study. Nucleotide sequence analysis was initiated because of the failure of in-house BPIV-3 RT-PCR methods to yield expected products for four of the isolates. Phylogenetic reconstructions based on the nucleotide sequences for the M-protein and the entire genome, using all of the available BPIV-3 nucleotide sequences, demonstrated that there were two distinct BPIV-3 genotypes (BPIV-3a and BPIV-3b). These newly identified genotypes have implications for the development of BPIV-3 molecular detection methods and may also impact on BPIV-3 vaccine formulations.

Journal

Journal of General Virology

Publication Name

N/A

Volume

ISBN/ISSN

1465-2099

Edition

N/A

Issue

Pages Count

Location

N/A

Publisher

Society for General Microbiology

Publisher Url

N/A

Publisher Location

N/A

Publish Date

N/A

Url

N/A

Date

N/A

EISSN

N/A

DOI

10.1099/vir.0.2008/000026-0