Evaluation of colorimetric detection methods for Shigella, Salmonella, and Vibrio cholerae by loop-mediated isothermal amplification

Journal Publication ResearchOnline@JCU
Soli, Kevin W.;Kas, Monalisa;Maure, Tobias;Umezaki, Masahiro;Morita, Ayako;Siba, Peter M.;Greenhill, Andrew R.;Horwood, Paul F.
Paul Horwood
Abstract

We evaluated loop-mediated isothermal amplification end-point detection methods for Salmonella, Shigella, and Vibrio cholerae. Detection sensitivities were comparable to real-time PCR methods. The colorimetric dyes hydroxynaphthol blue and SYBR Green I showed increased sensitivity when compared to visual and automated turbidity readings. End-point colorimetric dyes promise great utility in developing settings.

Journal

Diagnostic Microbiology and Infectious Disease

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Volume

77

ISBN/ISSN

1879-0070

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Issue

4

Pages Count

3

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Publisher

Elsevier

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EISSN

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DOI

10.1016/j.diagmicrobio.2013.09.009