Protein A as a fusion partner for the expression of heterologous proteins in Lactobacillus
Journal Publication ResearchOnline@JCUAbstract
An expression system based on the Staphylococcus aureus protein A gene (spa) was developed to allow the production and export of proteins in Lactobacillus. Plasmid shuttle vectors were constructed that carried the eZZ gene, a synthetic gene based on the Protein A gene (spa) but lacking the carboxy-terminal membrane-anchoring region. A gene fusion was created between the eZZ gene and the VD4 region of a chlamydial major outer-membrane protein gene. Expression studies demonstrated the recognition of the spa regulatory signals by several Lactobacillus, with the recombinant protein being expressed (from 0.1 mu g of EZZVD4 fusion protein per mi in L. plantarum up to 10 mu g of EZZ protein per mi in L. fermentum) and exported (levels up to 20% in L. fermentum) in several Lactobacillus strains.
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Volume
47
ISBN/ISSN
1432-0614
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Issue
5
Pages Count
6
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Publisher
Springer
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DOI
10.1007/s00253005096