Building a plant DNA barcode reference library for a diverse tropical flora: an example from Queensland, Australia

Journal Publication ResearchOnline@JCU
Costion, Craig M.;Lowe, Andrew J.;Rossetto, Maurizio;Kooyman, Robert M.;Breed, Martin F.;Ford, Andrew;Crayn, Darren M.
Abstract

A foundation for a DNA barcode reference library for the tropical plants of Australia is presented here. A total of 1572 DNA barcode sequences are compiled from 848 tropical Queensland species. The dataset represents 35% of the total flora of Queensland's Wet Tropics Bioregion, 57% of its tree species and 28% of the shrub species. For approximately half of the sampled species, we investigated the occurrence of infraspecific molecular variation in DNA barcode loci rbcLa, matK, and the trnH-psbA intergenic spacer region across previously recognized biogeographic barriers. We found preliminary support for the notion that DNA barcode reference libraries can be used as a tool for inferring biogeographic patterns at regional scales. It is expected that this dataset will find applications in taxonomic, ecological, and applied conservation research.

Journal

Diversity

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Volume

8

ISBN/ISSN

1424-2818

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Issue

1

Pages Count

5

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Publisher

MDPI

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EISSN

N/A

DOI

10.3390/d8010005