Development of genome-wide microsatellite genetic resources in a commercially important African freshwater fish species – the Nile perch, Lates niloticus

Journal Contribution ResearchOnline@JCU
Basiita, Rose K.;Zenger, Kyall R.;Mwanja, Matthew T.;Jerry, Dean R.
Dean Jerry
Abstract

[Extract] Nile perch, Lates niloticus, is a commercially important African freshwater species with limited available genetic resources. This study provides 24 polymorphic microsatellite markers for one of the most commercially important fisheries to the African continent validated in L. niloticus. Next-generation sequencing on Roche 454 GS-FLX instrumentation yielded ~122 000 sequences, which have been deposited at the NCBI (Accession no. SRP051308), and allowed the design of primers for 285 in silico perfect microsatellite loci (Table S1). Thirty-one microsatellite loci were tested for polymorphisms. Additionally, 16 microsatellites previously characterised for Lates calcarifer (a sister species) were tested in L. niloticus.

Journal

Animal Genetics

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Volume

46

ISBN/ISSN

1365-2052

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Issue

3

Pages Count

1

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Publisher

Wiley-Blackwell

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EISSN

N/A

DOI

10.1111/age.12283