Modulation of C-reactive protein and tumour necrosis factor-alpha in collagen-induced arthritis in Dark Agouti rats: Impact of collagen concentration on severity of arthritis

Journal Publication ResearchOnline@JCU
Tudave, Dulanthi;Radhakrishnan, Ammu;Chakravarthi, Srikumar;Haleagrahara, Nagaraja
Abstract

Objectives: The study investigated the effect of collagen-induced arthritis in Dark Agouti (DA) rats on the level of C-reactive protein and inflammatory cytokine tumour necrosis factor-alpha (TNF-α). Subjects: Female Dark Agouti (DA) rats. Methods: Three different dosages of (2 mg/kg of body weight, 3 mg/kg of body weight and 4 mg/kg of body weight) collagen and complete Freund’s adjuvant suspension were tested. After 45 days, serum C-reactive protein, TNF-α, superoxide dismutase and total glutathione assays were done. Radiographic and histopathological changes in the joints were compared. Results: All three groups showed signs of arthritic changes, confirmed by histopathological and radiographic changes. Severe arthritic changes were seen in the rats injected with 4 mg/kg of body weight of collagen. There was a significant increase in C-reactive protein, TNF-α, super oxide dismutase and total glutathione levels in the plasma in arthritis rats and the changes were more significant with 4 mg/kg of collagen. Conclusion: These results demonstrated that the optimal dose to inject to experimental animals in order to get server arthritic changes was 4 mg/kg of collagen with complete Freund’s adjuvant suspension. Severe arthritis changes induced significant elevation in plasma C-reactive protein and TNF-α levels.

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60

ISBN/ISSN

1420-908X

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Issue

10

Pages Count

11

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Birkhauser Basel

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DOI

10.1007/s00011-011-0349-y