Bismuth(III) beta-thioxoketonates as antibiotics against Helicobacter pylori and as anti-leishmanial agents

Journal Publication ResearchOnline@JCU
Andrews, Philip C.;Blair, Victoria L.;Ferrero, Richard L.;Junk, Peter C.;Kedzierski, Lukasz;Peiris, Roshani M.
Abstract

Nine different β-thioxoketones of general formula R1C([double bond, length as m-dash]O)CH2C([double bond, length as m-dash]S)R2 (R1 = C6H5, R2 = C6H5L1; R1 = C6H5, R2 = p-CF3C6H4L2; R1 = p-MeOC6H4, R2 = C6H5L3; R1 = p-MeOC6H4, R2 = p-CF3C6H4L4; R1 = C5H4N, R2 = C6H5L5; R1 = p-IC6H4, R2 = C6H5L6; R1 = C6H5, R2 = p-IC6H4L7; R1 = C6H5, R2 = C10H7L8 and R1 = CH3, R2 = C6H5L9) and their tris-substituted bismuth(III) complexes having the general formula [Bi{R1C([double bond, length as m-dash]O)CHC([double bond, length as m-dash]S)R2}3] were synthesised and fully characterised. The solid state structure of [Bi{C5H4NC([double bond, length as m-dash]O)CHC([double bond, length as m-dash]S)C6H5}3] B5 was determined by crystallography and revealed that the three β-thioxoketonato ligands are bound to bismuth(III) centre in a bidentate fashion through O and S atoms. The bismuth(III) complexes and the corresponding thioxoketones were assessed for their activity against H. pylori. All of the bismuth(III) complexes were highly active against H. pylori having a MIC of greater than or equal to 3.125 µg mL−1, while the free acids were essentially not toxic to the bacteria. The anti-leishmanial activity of all the bismuth(III) β-thioxoketonates and the corresponding free acids were assessed against L. major promastigotes. The toxicity towards human fibroblast cells was also assessed. All of the free β-thioxoketones were selectively toxic to the L. major promastigotes displaying some potential as anti-leishmanial agents. Among these [C6H5C([double bond, length as m-dash]O)CH2C([double bond, length as m-dash]S)C6H5] L1 and [C5H4NC([double bond, length as m-dash]O)CH2C([double bond, length as m-dash]S)C6H5] L5 showed comparable activity to that of Amphotericin B, killing about 80% of the L. major promastigotes at a concentration of 25 µM (6.0 µg mL−1). The bismuth(III) β-thioxoketonate complexes were toxic to both the L. major promastigotes and fibroblast cells at high concentrations, but gave no improvement in anti-leishmanial activity over the free β-thioxoketones.

Journal

Dalton Transactions

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Volume

43

ISBN/ISSN

1477-9234

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3

Pages Count

13

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Royal Society of Chemistry

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DOI

10.1039/c3dt52544a