Microsatellite markers in the Australian desert plant, Solanum centrale (Solanaceae)

Journal Publication ResearchOnline@JCU
Waycott, Michelle;Jones, Brendan L.;Van Dijk, Jent Kornelis;Robson, Heather L.A.;Calladine, Ainsley
Abstract

Premise of the study: The first microsatellite primers were developed for Solanum centrale, a native Australian species used in the bush foods industry. Existing markers developed for other Solanum species were also amplified. Methods and Results: Using an enrichment cloning protocol, seven novel markers were developed, and 48 existing markers from other Solanum species were tested, resulting in the characterization of a set of six highly polymorphic co-dominant loci for use in S. centrale. Microsatellite screening revealed polyploidy. Among the six highly polymorphic loci, allelic diversity ranged from 7 to 14. Conclusions: These markers will be useful for investigating genetic diversity and as a simple way of estimating ploidy of wild populations.

Journal

American Journal of Botany

Publication Name

American Journal of Botany

Volume

98

ISBN/ISSN

1537-2197

Edition

N/A

Issue

4

Pages Count

3

Location

N/A

Publisher

Botanical Society of America

Publisher Url

N/A

Publisher Location

N/A

Publish Date

N/A

Url

N/A

Date

N/A

EISSN

N/A

DOI

10.3732/ajb.1000356