Development and characterization of eight new microsatellite markers for the haremic sandperch, Parapercis cylindrica (family Pinguipedidae)

Journal Publication ResearchOnline@JCU
Smith-Keune, C.;Jerry, D.R.;Evans, B.S.;McCormick, M.I.;Munday, P.L.;Jones, G.P.
Dean Jerry
Abstract

Eight di-, tri- and tetranucleotide microsatellite markers were developed for the haremic sandperch Parapercis cylindrica using a linker-ligated, magnetic bead enrichment protocol. Screening of at least 17 individuals showed these markers to be polymorphic with observed heterozygosity ranging from 0.381 to 1.000 (mean = 0.742) and the numbers of alleles ranging from three to 18. The average polymorphic information content for these eight loci was 0.723. These markers may be used for parentage studies aimed at exploring the complex mating strategies employed by this haremic coral reef fish and will be valuable for population genetic studies.

Journal

Molecular Ecology Resources

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6

ISBN/ISSN

1755-0998

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Issue

4

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Publisher

Wiley-Blackwell

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EISSN

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DOI

10.1111/j.1471-8286.2006.01421.x