Development and characterization of microsatellite markers for parentage analyses of the coral reef damselfish (Pomacentrus amboinensis: Pomacentridae)
Journal Publication ResearchOnline@JCUAbstract
Five new polymorphic microsatellite loci were developed for the coral reef damselfish Pomacentrus amboinensis. Twenty-four individuals from two Great Barrier Reef populations were genotyped at the five loci, with numbers of alleles per locus ranging from 6–23 and observed heterozygosity between 0.42–0.92. In addition, the cross-species testing of six primers developed for Stegastes partitus revealed one primer (SpGATA40) that was also polymorphic for P. amboinensis. Due to high levels of polymorphism (≥14 alleles) in at least four of the six loci and a high proportion of tetranucleotide repeats, these microsatellite markers should be useful for parentage assignment as well as other investigations of individual relatedness.
Journal
Conservation Genetics
Publication Name
N/A
Volume
8
ISBN/ISSN
1572-9737
Edition
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Issue
4
Pages Count
4
Location
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Publisher
Springer
Publisher Url
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Publisher Location
Dordrecht, Netherlands
Publish Date
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Url
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Date
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EISSN
N/A
DOI
10.1007/s10592-006-9208-8