Monitoring of avian influenza in samples collected from aquatic birds in North Queensland (Old ID 27162)
Role
Chief Investigator
Description
At least 576 fresh faecal samples from wild ducks and magpie geese will be collected as environmental samples in the Townsville region. Swabs in transport media and immediately transported to the laboratory. Samples will be pooled and nucleic acid extracted. The extracted nucleic acid will be screened using assays based on TaqMan that will detect avian influenza. Pools that react will be individually extracted and tested. Avian influenza isolates will be checked using TaqMan specific for H5 and H7. Should the samples react in these assays it will be sent to AAHL for further testing and the CVO will be notified. Sequencing on all isolates other than those reacting in H5 and H7 will be carried out to determine genotype and to confirm pathotype.
Date
01 Mar 2021 - 05 Feb 2024
Project Type
GRANT
Keywords
RT‐PCR;Molecular Biology;Viral Diseases;Influenza;Avian;Wildlife
Funding Body
Wildlife Health Australia
Amount
37164
Project Team
Paul Horwood