Anti-inflammatory small molecules from Trichuris muris and Ancylostoma caninum
Role
Supervisor
Description
This project aims to isolate novel anti-inflammatory drug leads from parasitic helminths. All crude extracts from the helminth will be subjected to preliminary screening to determine their anti-inflammatory activity using an in vitro human peripheral blood mononuclear cell (PBMC) assay. Extracts or fractions showing the best anti-inflammatory activity will be selected for further purification and characterization of pure compounds. Pure compounds that have not been tested before for anti-inflammatory properties will be initially tested for cytotoxicity, and non-toxic compounds will be subsequently tested for anti-inflammatory activity using a PBMC assay with at least two different blood donors. Based on PBMC results, the most promising anti-inflammatory compound(s) will be further validated in vivo using a TNBS (trinitrobenzene sulfonic acid)-induced acute colitis mouse model.
Date
01 Jan 2024 - 31 Dec 2024
Project Type
GRANT
Keywords
antiinflammatory;pro-inflammatory;small molecules;cytokines
Funding Body
Far North Queensland Hospital Foundation
Amount
5000
Project Team
Phurpa Wangchuk;Alex Loukas