Projects

2

Publications

29

Awards

7


Contact Details

Lecturer, Microbiology

    Biography

    Dr Yaoqin Hong is a Lecturer in Microbiology at James Cook University, based in Townsville, Australia. His research explores how bacteria construct and maintain their complex cell envelopes—structures that underpin microbial survival, adaptation, and pathogenicity—and how these systems can be exploited for novel antimicrobial strategies.

    Yaoqin completed his PhD in Microbiology at the University of Sydney in 2014 under Professor Peter Reeves FAA, where he uncovered the role of membrane flippases as fidelity checkpoints in lipid-linked oligosaccharide biosynthesis, advancing how the diversity of bacterial surface polysaccharides is evolved. He then undertook postdoctoral training in bacterial lipid metabolism with Professor John Cronan NAS at the University of Illinois at Urbana-Champaign, before establishing his independent research program at Queensland University of Technology with Associate Professor Makrina Totsika FASM.

    His current work focuses on the biosynthesis and stability of the Gram-negative cell envelope, with particular interest in biofilm formation, membrane lipid homeostasis, and surface polysaccharide assembly. He also investigates innovative antimicrobial approaches that target these crucial pathways required for survival or virulence.

    Yaoqin has published ~ 30 research papers, in leading journals such as PNASMolecular MicrobiologyEcoSal PlusmLifeiScience, and multiple ASM and SGM society journals. He is a Fellow of the Australian Society for Microbiology, serves on the editorial role of Microbiology Spectrum, and invited reviewer board for international funding bodies. 

    He maintains active collaborations in Australia and internationally, including with the University of Illinois at Urbana-Champaign (USA), the Max Planck Institute of Colloids and Interfaces (Germany), the National University of Singapore, and several Chinese institutions such as South China Agricultural University and the Chinese Academy of Sciences.

    Research

    Research Interests

    Yaoqin Hong investigates the intricate mechanisms of bacterial cell envelope biogenesis and its central role in antimicrobial resistance and biofilm development. The cell envelope is a vital, multi-layered barrier that enables essential processes such as nutrient uptake and waste export. In Gram-negative bacteria, it consists of an inner phospholipid membrane, a rigid peptidoglycan layer, and an outer membrane, an asymmetric lipid bilayer with phospholipids in the inner leaflet and lipopolysaccharide (LPS) in the outer leaflet—that provides formidable protection against antibiotics.

    Bacterial surfaces are further encapsulated by polysaccharide structures, including O antigens, capsules, and the enterobacterial common antigen. These assemblies not only defend against host immunity and bacteriophage predation but also contribute to envelope integrity and homeostasis. Understanding how these envelope components are synthesized, assembled, and coordinated during growth is key to developing new strategies that destabilise the barrier, enhance antimicrobial susceptibility, and combat infections.

    Despite its significance, many aspects of envelope formation remain unresolved. Hong’s research aims to clarify these processes and translate this knowledge into novel antimicrobial approaches.

    Key Research Questions

    1. How do major cell envelope components interact, and how is lipid synthesis spatially and temporally coordinated with LPS and protein assembly during bacterial growth?

    2. What functional, metabolic, and regulatory networks govern cell surface polysaccharide encapsulation?

    3. What are the molecular and functional bases underpinning the structural diversity of bacterial surface polysaccharides?

    Projects

    Teaching

    Research Advisor Accreditation
    Advisor Type

    Primary


    Taught Modules
    Current JCU Research Students
    Innovative Poly (Lactic Acid) Nanocomposite for Sustainable Prevention of Marine Biofouling
    Doctor of Philosophy (Engineering and Related Technologies)
    Phytosynthesis and Characterization of Cerium Oxide Nanoparticles for visible light driven Photocatalytic and Antimicrobial applications
    Doctor of Philosophy (Natural and Physical Sciences)

    Activities

    Education
    Associations
    Awards
    Employment
    Committees

    Output

    Hong, Yaoqin; Qin, Jilong; Doyle, Matthew Thomas; Reeves, Richard (2025) 'Sequestration of dead-end . Microbiology, 171 (1). [DOI] ...
    Journal Publication Open Access ResearchOnline@JCU
    Jilong Qin; Yaoqin Hong; Waldemar Vollmer; Renato Morona; Makrina Totsika (2025) 'Disruptions in out. mBio, . [DOI] ...
    Journal Publication Open Access ResearchOnline@JCU
    Qin, Jilong; Hong, Yaoqin; Maczuga, Nicholas T.; Morona, Renato; Totsika, Makrina (2025) 'Tolerance . PLoS Gen, 21 (1)...
    Journal Publication Open Access ResearchOnline@JCU
    Penning, Stephanie; Hong, Yaoqin; Cunliffe, Taylor; Hor, Lilian; Totsika, Makrina; Paxman, Jason J.;...
    Journal Publication Open Access ResearchOnline@JCU
    Qin, Jilong; Hong, Yaoqin; Totsika, Makrina (2024) 'Determining glycosyltransferase functional order. Applied and Environmental Microbiology, 90 (6). [DOI] ...
    Journal Publication Open Access ResearchOnline@JCU
    Hong, Yaoqin; Qin, Jilong; Mitchell, Lachlan; Paxman, Jason J.; Heras, Begoña; Totsika, Makrina (202...
    Journal Publication Open Access ResearchOnline@JCU
    Hong, Yaoqin; Hu, Dalong; Verderosa, Anthony D.; Qin, Jilong; Totsika, Makrina; Reeves, Peter R. (20...
    Journal Publication ResearchOnline@JCU
    Qin, Jilong; Hong, Yaoqin; Morona, Renato; Totsika, Makrina (2023) 'O antigen biogenesis sensitises . PLoS Genetics, 19 (10). [DOI] ...
    Journal Publication Open Access ResearchOnline@JCU
    Hong, Yaoqin; Qin, Jilong; Forga, Xavier Bertran; Totsika, Makrina (2023) 'Extensive Diversity in Es. Microbiology Spectrum, 11 (4). [DOI] ...
    Journal Publication Open Access ResearchOnline@JCU
    Petit, Guillaume A.; Hong, Yaoqin; Djoko, Karrera Y.; Whitten, Andrew E.; Furlong, Emily J.; McCoy, ...
    Journal Publication Open Access ResearchOnline@JCU
    Qin, Jilong; Hong, Yaoqin; Morona, Renato; Totsika, Makrina (2022) 'Cysteine-Dependent Conformationa. Microbiology Spectrum, 10 (6). [DOI] ...
    Journal Publication Open Access ResearchOnline@JCU
    Qin, Jilong; Hong, Yaoqin; Pullela, Karthik; Morona, Renato; Henderson, Ian R.; Totsika, Makrina (20...
    Journal Publication Open Access ResearchOnline@JCU
    Hong, Yaoqin; Qin, Jilong; Verderosa, Anthony; Hawas, Sophia; Zhang, Bing; Blaskovich, Mark A.T.; Cr...
    Journal Publication ResearchOnline@JCU
    Dhouib, Rabeb; Vagenas, Dimitrios; Hong, Yaoqin; Verderosa, Anthony D.; Martin, Jennifer L.; Heras, ...
    Journal Publication Open Access ResearchOnline@JCU
    Subedi, Pramod; Paxman, Jason J.; Wang, Geqing; Hor, Lilian; Hong, Yaoqin; Verderosa, Anthony D.; Wh...
    Journal Publication ResearchOnline@JCU
    Verderosa, Anthony D.; Dhouib, Rabeb; Hong, Yaoqin; Anderson, Taylah K.; Heras, Begona; Totsika, Mak...
    Journal Publication Open Access ResearchOnline@JCU
    Hong, Yaoqin; Cunneen, Monica M.; Reeves, Peter R. (2019) 'Two extremely divergent sequence forms of. FEMS Microbiology Letters, 366 (8). [DOI] ...
    Journal Publication ResearchOnline@JCU
    Hong, Yaoqin; Liu, Michael A.; Reeves, Peter R. (2018) 'Progress in our understanding of Wzx flippas. 200 (1). [DOI] ...
    Journal Publication ResearchOnline@JCU
    Cao, Xinyun; Hong, Yaoqin; Zhu, Lei; Hu, Yuanyuan; Cronan, John E. (2018) 'Development and retention. Proceedings of the Nati, 115 (4):647-655. [DO ...
    Journal Publication ResearchOnline@JCU
    Hong, Yaoqin; Reeves, Peter R. (2016) 'Model for the controlled synthesis of O-antigen repeat units . mSphere, 1 (1). [DOI] ...
    Journal Publication Open Access ResearchOnline@JCU
    Hong, Yaoqin; Morcilla, Vincent A.; Liu, Michael A.; Russell, Elsa L.M.; Reeves, Peter R. (2015) 'Th. ...
    Journal Publication ResearchOnline@JCU
    Liu, Michael A.; Stent, Thomas L.; Hong, Yaoqin; Reeves, Peter R. (2015) 'Inefficient translocation . FEMS Microbiology Letter, 362 (9). [DOI] ...
    Journal Publication ResearchOnline@JCU
    Hong, Yaoqin; Reeves, Peter R. (2014) 'Diversity of O-Antigen Repeat Unit Structures Can Account for. 196 (9):1713-1722. [DOI] ...
    Journal Publication ResearchOnline@JCU
    Hong, Yaoqin; Duda, Katarzyna A.; Cunneen, Monica M.; Holst, Otto; Reeves, Peter R. (2013) 'The WbaK. Microb, 159 ...
    Journal Publication ResearchOnline@JCU
    Hong, Yaoqin; Cunneen, Monica M.; Reeves, Peter R. (2012) 'The Wzx translocases for Salmonella enter. Molecular microbiology, 84 (4):620-630. [DOI] ...
    Journal Publication ResearchOnline@JCU
    Hong, Yaoqin; Qin, Jilong; Totsika, Makrina (2025) 'Not too rigid nor too wobbly: Defining an optimal mLife, . [DOI] ...
    Journal Contribution Open Access ResearchOnline@JCU
    Hong, Yaoqin (2025) 'Decoding gene essentiality in Streptococcus suis using Tn-seq and genome-scale Microbiology Spectrum, . [DOI] ...
    Journal Contribution Open Access ResearchOnline@JCU
    Hong, Yaoqin; Qin, Jilong; Totsika, Makrina (2023) 'A conditional nature for the synthetical lethali Proceedings of the National Academy of Sciences, 120 (52) . [DOI] ...
    Journal Contribution Open Access ResearchOnline@JCU